An Adipocyte Cell Culture Model to Study the Impact of Protein and Micro-RNA Modulation on Adipocyte Function

Alteration of adipocyte function contributes to the pathogenesis metabolic diseases including Type 2 diabetes and insulin resistance. This highlights need better understand molecular mechanism involved in dysfunction develop new therapies against obesity-related diseases. Modulating expression proteins micro-RNAs adipocytes remains highly challenging. paper describes a protocol differentiate murine fibroblasts into mature modulate through reverse-transfection using small-interfering RNA (siRNA) micro-RNA mimicking (miR mimic) oligonucleotides. involves incubation transfection reagent oligonucleotides form complex cell culture plate which are added. The then allowed reattach adherent surface presence oligonucleotides/transfection complex. Functional analyses such as study signaling, glucose uptake, lipogenesis, lipolysis can be performed on transfected 3T3-L1 impact protein or manipulation function.